FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15881658
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA55350.016.6122361
GTACTAG21400.016.4759521
GTCCTAG39750.016.348761
GTGCTAG40950.016.0385021
GTCCTAA37150.015.1667621
GTATTAG32700.015.010831
GTCTTAG37100.015.0008561
GTTCTAG44900.014.9354911
GCTCTAG45600.014.023971
CTATACT37900.013.9381324
GTACTAA31000.013.8268771
GGTCTAG29150.013.7557261
GTATAGG27500.013.5754791
GTACAGG71200.013.5452891
GTCTTAA48250.013.5403091
GGTTTAG38150.013.138251
GTGTTAG39050.012.9239681
GTTCTAA49350.012.8182291
TCTATAC36400.012.6165973
GTATAGA37550.012.5196751
Produced by FastQC (version 0.11.5)