FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15567794
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33800.016.5490191
GTCCTAG38000.016.0828721
GTACTAG19100.015.9082791
GTACTAA33300.015.6569331
GTATTAA52800.015.4330591
TAGTGCG8850.015.43214857
GTGCTAG39150.014.5521161
GGTCTAA27700.014.2101621
GTATATA64100.013.7897341
GGTCTAG31900.013.7463371
GTTCTAG44900.013.5344391
GTGTTAG40600.013.26699351
CTATACT38000.013.266144
GTCTTAA45550.013.11389351
GCTCTAG45500.013.0524181
GTCTTAG37500.012.9826041
GTCCTAT33850.012.954451
TAGTCGG12000.012.9464949
TCTATAC33800.012.7693053
TATACTG48450.012.6854055
Produced by FastQC (version 0.11.5)