FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14934092
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG26700.016.4839291
GTATTAA40300.015.7844211
GTGCTAG34650.015.6789081
GTCCTAG35400.015.5409911
GTATATA49450.015.4364941
TATACTG42000.015.1455285
GTACTAG16500.015.0041191
GTACTAA26900.014.6996691
GTATATG42300.014.3878171
GTTCTAG38500.014.28963851
GTCTTAG32400.014.2207251
GGTCTAA27250.013.8799581
GCGATCG6000.013.75557437
CTATACT33150.013.6915434
GTACTAT30000.013.6391631
GGTCTAG27050.013.2198961
GTCTTAA37250.013.1076271
TTATACT48650.013.0752764
GTTCTAA40800.012.8941661
GTATTAT39700.012.8183811
Produced by FastQC (version 0.11.5)