FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14849429
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29550.016.4045181
GTACTAG17900.015.5572861
GTATATA45000.015.5090571
GTCCTAG34250.015.1571741
GTCCTAA29150.014.7425751
GTATTAA42750.014.5560761
GTTCTAG42450.014.415981
TTATACT46750.013.8254134
GTGCTAG34400.013.791851
TATACTG42650.013.7841175
GTACTAA23800.013.7229831
CTTATAC31150.013.6856523
GTACAGG67450.013.5581861
GTTCTAA39800.013.4754541
CTATACT30750.013.3046584
GTATATG41650.012.9594481
TCAGTAC42800.012.9325453
CCTATAC23650.012.6470713
GTACATA39200.012.5415681
TCTGTAC61500.012.5220313
Produced by FastQC (version 0.11.5)