FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14849429
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG38100.015.752521
GTATTAA40100.015.6549891
GTTCTAG42800.015.634491
GTATTAG27200.015.2173191
CGAATCG6800.014.66212759
GTACTAA25500.014.6086251
GTATATA46800.014.5930171
GTTCTAA40200.014.4148121
GTATAGG23250.014.3904551
GTACTAG13000.014.3276891
TCTATAC29850.014.19153
GTGCTAG36250.013.9873381
GTCCTAA32250.013.9039881
GTATATG40400.013.8311851
CTTATAC32000.013.7761373
GGTCTAA26600.013.7451661
CTATACT33400.013.7032234
GGTCTAG28050.013.2805681
CTAAGCG9900.013.2088794
TATACTG43500.013.2032225
Produced by FastQC (version 0.11.5)