FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15117072
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29850.018.317221
GTACTAG17150.016.2415581
GTCCTAG36300.016.1993051
GTTCTAG41050.015.4976341
GTATTAA42150.015.2563561
GTTCTAA41500.015.1638621
GTGCTAG36550.014.6772281
CTAAGCG9600.013.9680424
GTACTAA26750.013.883751
TAGTGCG7100.013.86264357
GTATATA45850.013.5751941
GTATATG45400.013.4825161
CTAGACT31000.013.3095014
GTCTTAG30600.013.260731
GTACTAT30850.013.1532691
GTATAAG29100.013.117071
TCTGTAC62700.013.0512343
GTCTTAA38600.013.0068581
GTACAGG65650.012.99043751
TATACTG43250.012.9581695
Produced by FastQC (version 0.11.5)