FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867951
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36350.016.665251
GTACTAG17050.016.1498761
GTACTAA26250.015.8657151
GTATTAA41100.015.40918351
GTATTAG26500.015.3263861
GTATATA46600.015.2154541
GTTCTAG42450.014.7569741
CTATACT33400.014.7231784
TACGCGA2605.916809E-714.55544952
TATACTG43300.014.2948325
TCTATAC31450.014.2162273
GGTCTAA28400.014.0586381
CCTATAC22700.013.9387443
GTCCTAA29650.013.5820331
GTGCTAG36750.013.5804541
GTATTAT40300.013.5798721
GTTCTAA39150.013.4512691
GTCTTAA36600.013.3539841
GTACATA38900.013.2722731
GTGCTAA34550.013.2497341
Produced by FastQC (version 0.11.5)