FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12462480
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22800.017.6720431
GTCCTAG31950.016.9225031
GCGATCG6700.015.93564737
GTATTAA30550.015.7816571
GTACTAA23400.015.3057021
CGATCGT6550.015.24991438
GTCTTAG25300.015.2452061
GTATAAG23950.014.2352611
GTCCTAA24500.014.0562561
GTACTAG13450.013.826341
GTTCTAG35500.013.5811161
TTATACT35800.013.56399254
GTACATA33750.013.1629041
CTGTACT62500.012.9490924
GTCCTAT25800.012.94755651
TTGCGCG6650.012.94629212
GTATATA38800.012.8698071
GTTCTAA32250.012.7072931
TCTAGAC26500.012.6058993
GTCTAGG29600.012.5651551
Produced by FastQC (version 0.11.5)