FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12462480
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14600.018.914221
GTCCTAG31700.016.8781071
GTCTTAG27900.016.8261991
GTCCTAA26000.015.9315931
GTATTAG22900.015.5257661
GTTCTAG35150.015.5161161
GTACTAA21900.015.1313761
GTGCTAG30300.014.8098961
GCTCTAG37550.014.6163341
GTATTAA34700.014.3246591
CGATCGT6400.013.9924538
TACTAGG16700.013.8404812
GTTCTAA34650.013.7476081
GTATATA34900.013.5502231
GTATAGG17800.013.1868241
GTCTTAA30800.013.1125281
GCGATCG6850.013.07239837
GTCCTAT26700.012.9282581
CTCCTAG51200.012.7421631
GGTCTAA21700.012.7256971
Produced by FastQC (version 0.11.5)