FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12700612
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13950.018.2855821
GTATTAG24200.018.0900421
GTGCTAG29950.017.1490751
GTCTTAG27850.016.0904961
GTACTAA21550.015.5158531
GTCCTAG29700.015.2042881
GTATTAA31700.015.0062111
CGAATCG7950.014.7314289
GGTCTAA22700.014.5779551
GCTCTAG35450.014.1967141
GTTCTAA32500.014.1065081
GGTCTAG25950.014.0805541
CTAAGCG10850.013.9682064
GTCCTAA26200.013.814631
GTATACG5250.013.7883161
TCTATAC25450.013.6697273
CTATACT26250.013.6465144
GTACATG48750.013.5761881
TCTAGAC27200.013.423423
GTTCTAG33900.013.3205711
Produced by FastQC (version 0.11.5)