FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12733694
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG32150.017.365821
GTCCTAA28900.016.4566921
TAGTGCG6200.016.40351957
GTACTAG14600.016.0515631
GTCTTAG29350.015.9695281
GTATTAA36250.015.8770481
GTATTAG21550.015.0328681
GTTCTAA35950.014.6674231
GTATAAG24700.014.0924231
GTCTTAA30500.013.8984561
CTATACT27850.013.8510544
GTTCTAG34200.013.8056141
GCTCTAG34250.013.78545951
GTATAGG19100.013.71328451
GGTCTAA23650.013.5523011
GTGCTAG31500.013.2384191
GGTCTAG23250.013.0443061
CGATCGT5300.012.99799938
GTGCTAA31300.012.9926851
GTATATA39100.012.8687791
Produced by FastQC (version 0.11.5)