FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398453
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG750.002020349722.9474351
TTAGACG1200.001500014117.2109344
GTATTAG4000.017.2105751
ATACGGT1250.001971086316.52256
GCGCTAA1250.00197136116.5221521
GTACTAA3651.8189894E-1216.0317691
ATACTAG3805.456968E-1215.3989351
GTCTATA4600.014.9657171
GTATTAA6450.014.9425151
CTACGCT1851.0419824E-414.8851324
CTAGTCC3808.731149E-1114.4934184
CTAAGCG2152.3138587E-514.4091554
CCTATAC3605.984475E-1014.3424463
GTATATA7700.014.3048941
GTCTTAG5100.014.1734151
CTAGACT5600.014.1375534
GTATAAG4152.3646862E-1114.1002311
CGAGCGT2202.8801316E-514.07844416
GTTCTAG7100.014.0593431
TAGACTA2708.9983223E-714.0237245
Produced by FastQC (version 0.11.5)