FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374654
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2400.022.948461
GCGATCG1154.6878624E-520.95161637
CTAACGC950.00793886318.1172054
GCGTAAA1150.001127542317.9596611
CGATCGT1452.7700744E-416.6185538
GTCCTAA5250.016.3917561
GTACTAA4050.016.1489141
TAGTGCG1755.5177996E-516.03598257
GTATAAG3753.6379788E-1215.6049521
GTCTTAG4550.015.1308521
GCGTAGA2051.4650699E-515.12322942
GTATAGA4950.014.6035641
CGCGAGA1657.412104E-414.6004917
ATAGTAC3554.8567017E-1014.5447993
GTGCATA5500.014.3949431
GTACTAT4800.014.3427881
GTACATG9850.014.3282241
CTGTACT11150.013.8925654
ACTCGCG1750.001156521713.76646612
TCTGTAC8900.013.5370123
Produced by FastQC (version 0.11.5)