FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424362
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT1852.3570465E-718.85491454
CTTAACG1150.00112570117.9640623
CGTAGTA1150.00112570117.9640622
GTACTAG2208.780626E-817.2155591
GTATTAG4550.015.8912841
GGCGTAC1554.596001E-415.5495371
TAGAACG1350.003287897415.3027195
TTAAGCG1350.003287897415.3027194
TACACCG1808.230444E-515.3027195
GTCTAAT3201.7480488E-915.0636141
TTGCGCG1605.8622245E-415.05926512
GTGTTAG5750.014.9700511
GCCTTAT5750.014.9700511
GTTATAG5100.014.8526381
CGGTGCG2757.818376E-414.49737770
GTATAGG3808.731149E-1114.4973131
TATTAGG4650.014.0686272
GTCCTAG4505.456968E-1213.7724471
GGTCTAA5000.013.7724471
GTATTAA6500.013.7724461
Produced by FastQC (version 0.11.5)