FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362852
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA4150.019.9228481
CCTATAC3400.019.235543
TAATACG1108.3721644E-418.773774
GTGCTAG6150.017.3650041
GTACTAG3003.8198777E-1117.2249621
GTCCTAA5300.016.8999651
GTATTAA6000.016.6507971
GTTCTAA6000.015.5024661
GTCCTAG5850.015.3110791
CTCCTAG9300.014.8171721
GTATATG6750.014.800711
ATTATAC5000.013.7685963
GTCCTAT4851.8189894E-1213.4958491
GTATTAG3851.6189006E-913.42204951
CTATACA5150.013.3664394
TCTGCAC16000.013.3383283
GCCTTAG5450.013.2742831
CCTAGAC4153.5470293E-1013.2709373
GTTCATA6850.013.0758861
CTAACCC4509.276846E-1113.0025754
Produced by FastQC (version 0.11.5)