FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11542684
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18700.016.4001671
GTATTAG34750.015.2343451
GTTCTAG41150.014.7281711
GTCCTAG30350.014.4355441
GTCTTAG34850.013.72394851
GTACTAA28700.013.4844911
GCGAGAC47400.013.1700943
GTGCTAG36700.013.0321441
CGAGACT49800.012.9752284
GCTCTAG34500.012.9107471
CGAGACG13950.012.55895373
GTACGAG8050.012.2455571
GTACTAT29300.012.0868951
GTCCAGG84850.012.0480511
GTATAGG22200.011.6765891
GAGCGAG54100.011.675071
GTACAGG40850.011.6188161
GTCTAGG30700.011.53571
CCAGGAC87050.011.5328063
GTCCTAA32200.011.4518631
Produced by FastQC (version 0.11.5)