FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11542684
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21200.016.5219941
GTCCTAG32250.016.4045891
GCGAGAC48350.015.3186023
GTATTAG37350.015.1414741
GTGCTAG36650.014.4351431
CGAGACT48100.014.3363984
GCCCTAG29100.014.1681311
GTTCTAG43350.013.2982661
GCTCTAG32050.013.0917261
GTCTTAG31900.013.0389091
GCGCTAG4202.5229383E-913.030741
GGTCTAG24400.013.00937751
GAGCGAG55250.012.6793221
GTGCAGG76300.012.6242761
GTGTTAG43850.012.5641881
AGCGAGA55900.012.5315622
GTACTAA32250.011.992321
GGACCGT7750.011.7700066
GTCCAGG79250.011.7399971
CGACGAC15650.011.66416773
Produced by FastQC (version 0.11.5)