FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11436442
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19700.020.1842171
GTCCTAG34550.014.1484351
GTTCTAG43000.013.8283641
GCGAGAC44750.013.5329163
GTCTTAG32350.013.5318951
GTGCTAG37850.013.3004041
GTATTAG36000.013.27456951
GGTCTAG24050.013.196411
GTACTAA29850.013.0764871
CGAGACT45200.012.9947974
GGTCTAA26600.012.7541921
GAGCGAG50700.012.6635751
CTAGACC24250.012.486724
GCTAGAC22850.012.4533073
GCCCTAG26850.012.0919791
GTGCTGG187250.012.0397741
GTCCTAT29450.012.0153951
GTCCAGA52800.011.9526411
GTACAGG43700.011.9373041
GCTCTAG31750.011.8343711
Produced by FastQC (version 0.11.5)