FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11436442
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20150.016.4876521
GTCCTAG33350.015.6542651
GCGAGAC46800.014.7403493
CGAGACT48900.014.7045284
GTTCTAG39100.014.3792561
GTACTAA29950.014.0182391
CGAGACG13900.013.1299873
GTCCTAA34600.013.0839241
GTATTAG35150.012.9830631
GAGCGAG53300.012.9457451
CGCGATT2000.00203264812.77429961
GTCTTAG36900.012.5652111
AGCGAGA53750.012.4269472
GTGCTAG37300.012.1368311
GTCCAGG84550.012.1334731
GCTCTAG32300.012.0941041
GTATTAA56000.011.9956071
CCAGGAC83250.011.8378093
ATAGTAC27650.011.6166093
GGCCTAG33000.011.3950371
Produced by FastQC (version 0.11.5)