FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10521153
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17900.016.3177071
GCGAGAC43200.015.9688753
GTGCTAG35550.014.8919161
GTTCTAG39400.014.7340821
CGAGACT45150.014.7132934
GAGCGAG47650.014.3285051
GTCCTAG28500.014.2200231
AGCGAGA49800.012.9730552
GGCCTAG33350.012.8089111
GTCCTAA26150.012.7055031
GTCTTAG30550.012.6682571
GTATTAG33250.012.6278191
GTACAGG39100.012.6060541
GGTCTAG21800.012.5610791
GGTCTAA22800.012.3304261
CTAGACC22850.012.2998784
CCAGGAC81350.012.1592843
GTCCAGG83200.012.0678951
GTCTAAG26150.012.0073991
GTGCAGG71500.012.0000761
Produced by FastQC (version 0.11.5)