FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10521153
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19100.015.6702511
GTATTAG33350.015.6507681
GCGAGAC44400.014.5483433
CGAGACT44600.014.2378984
GTTCTAG37750.014.1166411
GTCCTAG31300.014.1103091
GTCCTAA27450.014.0948041
GTGCTAG32550.013.792711
GTCTTAG30300.013.6122931
GCCCTAG26500.012.9472481
GAGCGAG49350.012.8693791
GTATAAG28450.012.4447151
GTACTAA29350.012.4361921
AGCGAGA50200.012.4315692
GGTCTAG21700.012.2788761
GCTAGAC24100.012.1142413
GTCCAGG76800.011.9766351
CTAGACC21650.011.9682864
GTACGGG5902.1827873E-1111.7543081
GTGCTGG169100.011.4400451
Produced by FastQC (version 0.11.5)