FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10253024
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG28750.017.0195481
GCGAGAC44650.015.36848453
GTACTAG17750.015.2234971
GAGCGAG49300.014.2211721
CGAGACT45450.014.0539644
GCGCTAG3654.8839865E-914.0060681
AGCGAGA49600.013.7611462
GTGCTAG31800.013.3202381
GTTCTAG35950.013.3061831
GTCTTAG29850.013.0894241
GTACAGG40100.012.8397281
CGCTATA3455.2665746E-712.6957052
GGCCTAG32000.012.6664261
GTATTAG31550.012.38413051
GTGCAGA65500.012.3206061
GGTCTAG20900.012.2301811
GGTCAGG125300.012.1525131
CAGGACT61400.012.1270874
GTCCAGG75700.012.0111481
GCTCTAG29050.011.9414911
Produced by FastQC (version 0.11.5)