FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10253024
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17050.016.4824941
GTCCTAG31000.015.6583711
GCGAGAC42000.015.2060263
GTGCTAG30600.015.0281581
CGAGACT40500.014.4172134
GTATTAG32350.013.9895591
GTCTTAG29150.013.8976391
GAGCGAG46250.013.4940081
GGTCTAG21100.013.1458231
GTTCTAG36900.013.0558211
GTGCAGG68500.013.0003751
GCTCTAG28900.012.7549911
GCTAGAC23750.012.2928713
AGCGAGA48800.012.26483252
CTAGGGT24700.012.2630224
GTCCTAA26500.012.1197441
GTACAGG39500.012.10405351
GTCTAGG27350.011.6096341
GTACTAA29500.011.505821
GTCCAGG76500.011.4023161
Produced by FastQC (version 0.11.5)