FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6083302
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG17250.017.6225911
GTACTAA11250.016.2741971
GTCTTAG14550.015.9069891
GTTCTAA16400.014.9551471
GTCCTAG15700.014.5218011
GTATTAG12950.014.4045661
GTCTTAA16250.013.8177141
GGTCTAA9500.013.8177141
CACGCGA2002.0101656E-413.77841812
GTACTAG6550.013.71223451
GGTCTAG12400.013.6505641
GTGCTAA14250.013.5752971
CTAACGT2851.6278827E-613.3053544
GTGCTAG16200.013.2206511
TTGCGCG2651.13442675E-512.99850812
GTACGAA2952.3426674E-612.8809191
ATAGTAC9400.012.8426493
TTATACT19400.012.7940894
GTACTAT10300.012.74449351
GTATAGG8500.012.5985041
Produced by FastQC (version 0.11.5)