FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6034768
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11100.016.4506131
GTACTAG6550.016.3060821
TACGCTA1300.002545927415.9018585
GGTCTAA11000.015.6605321
GTCCTAG14300.015.4196011
GTATACG1808.18882E-515.3125221
GTTCTAG16200.014.8871731
GTACAGG26200.014.2020711
CTAGACC11350.013.9636324
GTATATA18800.013.9278771
GTATAAG11100.013.6571121
GTCTTAG14700.013.5937671
TTGCGCG2557.6252054E-613.50903212
TCTATAC12600.013.3985663
TATACGC2651.1317197E-513.001525
TATACTG18300.012.8025895
GTCCTAT11850.012.7927391
TCTAACC12700.012.7504923
GTACATA16300.012.6821491
GTATAGG9400.012.4617851
Produced by FastQC (version 0.11.5)