FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6123854
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7250.018.0550731
GTCTTAG14650.015.7539981
GTACTAA10300.015.0497381
CTAGCGT2302.61907E-614.9771554
GCGCTAA2202.8555107E-514.0920261
TCTATAC14200.014.0700883
TAGTGCG2758.909865E-714.03690957
GCTCTAG17300.013.7390471
GTTCTAA16850.013.2882281
GTATTAA17950.013.0496251
ACTAGCG1850.001746794913.0341723
GTCTTAA15600.013.0280981
GTATTAT18000.013.0133781
CTATACT13600.012.9177954
GTACAGG25650.012.8925091
GCGTTAG2153.7056624E-412.81755351
GTATTAG11850.012.7905121
GTCTAAG12400.012.7787911
TCTAGCG2701.3744964E-512.7583173
GTATAGG9750.012.7189561
Produced by FastQC (version 0.11.5)