FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6123854
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10900.019.9263151
GTACTAG6950.019.3460671
GTCCTAG14950.016.6036761
GTTCTAG16500.016.0886541
GTACCGT3006.9849193E-1016.072766
GTCTTAA15750.015.541421
GTCTTAG13750.015.0439361
GTCCTAT13400.014.6650311
TATACCG1951.623379E-414.1297835
GTATAAG12800.014.0057481
GTGTTAG16950.013.8309551
GTACAGG27050.013.5098821
TAGTACG1550.008206218513.332464
GGTCTAA12000.013.2156811
TAACACG5550.013.032184
GTACTAA10900.012.9679191
GCTCTAG16700.012.7993571
GTATTAA19800.012.7107341
CTAGACT13300.012.6892274
GTATTGG13350.012.6539981
Produced by FastQC (version 0.11.5)