FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5985727
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG14150.017.7704331
GTATTAG12800.016.9536111
GTACTAG6650.016.0572681
CTATACT13700.015.5887044
GTATTAA14950.015.2066791
CCGCTAG2554.6840069E-714.8588131
GTTCTAG16650.014.4815641
TATACTG16650.014.2750425
ACGATCG1500.005489156614.16735160
CTTATAC11650.013.6009993
GTGCTAG15800.013.2985531
GTCTTAA16300.012.67931
GCTATAC8450.012.6369963
CAGTACT21650.012.5692134
GTCTATA11200.012.3019391
GCCCTAG15700.012.2862691
GTATATA20550.012.236091
GCTCTAG17500.012.2035251
GTACAGA26300.012.1803241
GTACGAC4257.0813257E-912.1574143
Produced by FastQC (version 0.11.5)