FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834906
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18650.017.2150691
GTCCTAG37600.017.0777151
TATACCG4750.016.1304075
GTGCTAG36200.015.6216931
GTTCTAG42850.015.3259221
GTCTTAG36000.015.3030991
GTATTAG39050.014.8552871
GCGAGAC47700.014.8380663
CGAGACT47050.014.6555964
GTCCTAT30650.014.5222641
GGTCTAG29550.014.0751281
GTATAGG25200.014.0435261
GTCCTAA32750.014.0366731
GAGCGAG51700.013.8315381
GGTCTAA28400.013.7458121
GGTTTAG35900.013.719691
GTACTAA32450.013.7167141
GTGTTAG48500.013.5405321
GTGCTAA36800.013.3841541
TCTAGAC34750.013.3334613
Produced by FastQC (version 0.11.5)