FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11839176
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG26600.016.0580581
GTTCTAG44950.015.9189781
GCCCTAG32950.015.2901761
GCGAGAC46200.015.2476623
GTCCTAG34500.015.238151
GTATTAG38750.015.0742981
GTACTAA30600.015.0327341
CGAGACT46050.014.9804124
GTGCTAG37950.014.6224661
GTACTAG20550.014.3900391
GTATTAA56200.014.1614921
GTCTTAG36650.014.1450081
GTACAGG47050.014.1221421
GTCCTAA30450.013.5481491
CTAGACC27100.013.46859554
GAGCGAG51000.013.3147061
GTATTGG32750.013.2655271
AGCGAGA52300.013.1203092
CAAGACT91000.013.0356764
GTATAAG32000.013.0059971
Produced by FastQC (version 0.11.5)