FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13044778
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16400.015.1574491
GTCCTAG33600.014.5881541
GTATTAG25200.014.449221
GTACTAA22800.014.2810351
GTGCTAG35250.014.2032771
GTATTAA35000.013.704531
ATCTCCG152850.013.69333370
GTTCTAG39150.013.5932541
GTCTTAG29250.013.5258381
GTATATA47650.012.8583951
GGTCTAG27850.012.5714811
GTACAAG39600.012.4662421
GTATAGA27250.012.4628341
GCCCTAG34600.012.2439321
TCTCCGA129350.012.14935970
GTCTTAA35750.012.0459391
GTATTAT34100.012.0127681
GCTCTAG38100.011.9462061
TCAGTAC34650.011.9195683
GTGCTAA31050.011.7269041
Produced by FastQC (version 0.11.5)