FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12958973
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT5350.018.31787338
GTATTAG25300.016.3290651
GTCCTAG33800.015.537261
GCGATCG6700.015.14946737
GTACTAA21300.014.1357461
GTCTTAG29400.013.0992141
GTATTAA34650.013.0342591
GTGCTAG31750.013.0118231
GTATAAG24850.012.8207921
ATCTCCG152050.012.68306670
GGTTATA26650.012.6117081
GTCCTAA27700.012.5128231
GTATATA49250.012.5114121
GTACAAG40350.012.407731
GTATACG5751.8189894E-1212.17760851
GTTCTAG36000.012.1564671
GTTCTAA34100.012.1151141
TATACTG37400.012.0730625
TCTCCGA125700.011.889173570
GGTCTAG27500.011.8399571
Produced by FastQC (version 0.11.5)