FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12958973
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16600.016.8671051
GTACTAA23250.015.6555781
GGTCTAG26400.015.2458841
GTCCTAG32250.014.9764231
GTGCTAG34600.014.7684681
GTCTTAG28550.014.7107171
GTATTAG24600.014.369611
GTACATA36750.013.5235191
GTTCTAG39950.013.4915751
GTTCTAA36450.013.3467641
GTATTAA38400.013.1247181
GTATATA46900.013.0594211
TCTGACG119900.012.84470370
GTCCTAA27700.012.7614581
GTACAGG54700.012.2209441
TATACTG37200.012.0410335
GTCTATA24000.011.9580761
GTGCTAA31200.011.7785931
CTGACGC104350.011.7734970
TCTATAC26500.011.7512883
Produced by FastQC (version 0.11.5)