FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11732529
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG27900.016.8175531
GTATTAG21750.016.6555161
GTATAGG18350.016.1692711
GCTCTAG33550.016.1448941
CGATCGT5350.016.08883538
TACGCGA2402.3418397E-715.795822
GCGATCG6300.015.30083437
CTAAGCG8550.015.3001834
GTATTAA33050.015.2408591
GTTCTAG33150.015.1948831
TAGTGCG7000.015.04204357
GTACTAG13700.014.857971
GGTCTAA21600.014.3752971
TTGCGCG6500.014.29558212
GTACTAA22450.014.2920531
TATACTG35800.014.2291865
GTGCTAG28500.014.0423951
GTTCTAA32800.013.9896171
CGAATCG5700.013.8868699
GGTCTAG23500.013.8002841
Produced by FastQC (version 0.11.5)