FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11351290
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146810.12933331806340953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG6600.018.25485637
GTACTAG12250.018.024281
CGATCGT6300.017.48615638
GTGCTAG27700.016.1911471
GTCTTAG24100.015.7467031
GTTCTAG32000.015.3091961
GTCCTAG26800.015.0613931
GGTCTAG20050.014.1095621
GTACTAT19250.013.9790571
GTCCTAA23100.013.8894481
GTATTAG19900.013.86918451
GTGTTAG28000.013.799841
GTATATA37900.013.7452231
TATACTG31350.013.723525
GTGCTAA26650.013.5927121
GTATAGG20100.013.5595441
GTACTAA19850.013.5565171
GCTCTAG33000.013.4862071
GTCTTAA28200.013.457291
GTATAGA26150.013.4568211
Produced by FastQC (version 0.11.5)