FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14333897
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA28950.023.290243145
GTATTAG44000.021.7596451
GTCCTAG58350.021.753431
GTACTAG26450.020.8409961
CTATACT52900.019.3237974
CGTCTTG66100.018.317015145
CACGAGC92650.018.154413145
GTGCTAG59650.017.9962431
GTATTAA68950.017.7780041
GTATATA74650.017.5864961
TCTATAC50800.017.553773
TCTCCGC138100.017.324467145
ACACATA163050.017.119047145
GTATATG64900.016.98751
GTCTTAG53950.016.9398561
GTATAGG36550.016.8679431
GTACGAC18500.016.8510043
CATGATG292050.016.731787145
GTTCTAA66000.016.3746831
GCTCTAG74150.016.3356291
Produced by FastQC (version 0.11.5)