FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14333897
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15650.027.330954145
GTACTAA42000.021.7969321
TGACGCG24950.021.501972145
GTCCTAG60000.021.1914621
GTACTAG26200.020.7985971
CGCCGTT24400.020.79818145
TTATACC101300.020.539486145
GTTCTAG66850.018.9113251
GTATTAG46400.018.7904571
GCTCTAG66650.018.6410371
GTATTAA69850.018.2031151
GTCTTAG53600.017.8930021
GGTCTAA47650.017.2301731
GTTCTAA67600.016.874351
GTAGATA80800.016.32966145
CTATACT50650.016.0395454
GGATTAG53300.015.9489741
GTCCTAA48900.015.7496581
CTGCCGC245950.015.740247145
GTGCTAA54600.015.1700261
Produced by FastQC (version 0.11.5)