FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14661227
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG74950.024.762955145
GTACTAG28050.021.4571821
TCTCGTT64500.020.794388145
CGTCTTA32150.020.069807145
CACGAGC119650.019.995644145
ACACATA175150.019.951294145
GTATTAG44600.019.3481541
GTCCTAG59800.018.5531521
GTATAGG37700.018.2729951
TCGTATT41050.018.19107145
GTACTAA41400.018.0411551
TGCCGTG85200.017.699373145
GTCCTAA51850.017.6217581
TCTCCGC175700.017.247995145
GTGCTAG58600.017.2006421
TTATACC114350.017.118343145
CATGATG299800.017.048714145
GTGTAGG81750.017.031031
CGAGACA66300.016.949324145
GTATTAA69850.016.921881
Produced by FastQC (version 0.11.5)