FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14401698
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA36750.024.659525145
CGTCTTG70600.023.002518145
GTATAGG37500.020.1056231
GTATATA71850.020.0789831
CTCCGAC71950.019.548029145
GTACTAG27350.019.351
GTCCTAG58750.019.1266651
GTATTAG45150.018.78641
GTACTAA42400.017.9530771
ACACATA174350.017.506212145
CGAGACA62350.017.092789145
CACGAGC127400.016.958166145
GTTCTAG65550.016.3683341
GTGCTAG60250.016.3642941
GTATAGA50200.016.3188741
GCTCTAG74350.016.1861131
TCTCCGC193200.015.723128145
TCTCGTT61750.015.497762145
GTACATA59450.015.4870021
CATGATG300550.015.462273145
Produced by FastQC (version 0.11.5)