FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14401698
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT24150.032.42831145
CCGTATA21500.030.01714145
GTCCTAG60100.024.6161541
TGACGCG34000.021.114168145
GTATTAG48200.020.7633081
GTTCTAG65550.019.6929951
GTACTAG25600.019.5467071
TTATACC129250.018.626244145
GTGCTAG55850.018.4386731
GTCTTAG52500.018.3720171
GTAGATA103050.018.365816145
GCTCTAG75800.017.12571
GTCCTAA50100.016.936071
GTCTTAA69850.016.81951
GTACTAA44700.016.5484451
GTATTAA71550.016.5212331
GCGCTAG12350.016.4420361
GTCCTAT49150.016.3781171
TAGATCG21850.015.929698145
GTATATG67200.015.6481571
Produced by FastQC (version 0.11.5)