FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8299980
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14150.019.990971
GTCCTAG19600.017.2483041
GTCTTAG16400.014.5138181
GTACTAG9150.013.9494491
GTTCTAA22100.013.5801131
GTATAAG15500.013.5760851
CTATACT18900.013.5075864
GTCCTAT19000.013.4355231
GTCTTAA22500.013.338691
GTTCTAG23100.013.2909021
GTATTAA23550.013.1834181
TCGTGCG3402.9796932E-413.15257270
GGTCTAG15800.012.8816451
GTACTAA14300.012.5442221
GTATAGG12950.012.5200051
GTATTGG19600.012.4962211
GTTCTAT21450.012.3833981
CTAGACC16500.012.3360434
TCTATAC17550.012.1873513
GTATAGA17000.011.9723541
Produced by FastQC (version 0.11.5)