FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8299980
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10050.018.9302711
GTACTAA14200.018.7569661
GTCCTAG19950.018.3790531
GTATTAG15400.016.3969231
GGTCTAG15550.016.0163041
GTGCTAG18600.015.4356651
CGAATCG3402.382876E-1015.24727745
GTATATA27100.015.1893031
GTGTTAG20950.014.8599951
CTTAACG5600.014.1874673
CGATCGT3657.1304385E-1014.17940738
GGTCTAA14200.013.8850261
GTACTAC13050.013.7832951
GTCTTAG18700.013.6883241
GTTCTAA23550.013.5131661
GTATTAA25650.013.0811091
GTCTATA15100.013.0574431
GTACAGG37400.013.0409031
GTATAAA31050.013.0342031
GTTCTAG22950.012.8113961
Produced by FastQC (version 0.11.5)