FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8316051
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG19250.019.3559571
GTATTAG15700.019.1179451
GTCTTAG17350.016.5044171
GTACTAG9950.014.9096361
GTGCTAG17550.014.7436771
GTATATG22550.014.5344531
GTATTAA26000.014.4635461
GCTCTAG23100.013.8896921
GTATAAG16400.013.8842281
GTATATA24750.013.5212911
GTCTTAA21750.012.689731
GGTCTAG14300.012.3042681
GTATAGG12700.011.9528261
TAGGGTG18850.011.89726455
GTTCTAG20600.011.8908461
GTCTAAG18400.011.8125691
GCCCTAG20000.011.7300681
CTACTAG11650.011.5494241
GTCTAGG19750.011.52918151
CTAGCGC4207.662675E-811.5004834
Produced by FastQC (version 0.11.5)