FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8602408
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG20700.018.0139261
GTACTAG9800.016.5587181
GTATTAG15450.016.3135811
GTTCTAG22950.016.097411
GTACTAA15500.015.5926981
GTATTAA24000.015.2492881
GTTCTAA23800.013.9267321
GCTCTAG22850.013.9013371
CGAATCG4355.0931703E-1113.4807199
TCTATAC16250.013.3745273
GTATACG3651.07211235E-813.24311351
CTATACT18700.012.912864
GTGCTAG19100.012.8345281
GTCTTAG16250.012.7483171
CTAGACC15750.012.7032084
GTCTAGA17150.012.6832741
GTACAGG37450.012.5383971
GTATATG24050.012.4899041
GTACAAG31450.012.4054561
CTAAGCG5900.012.2781594
Produced by FastQC (version 0.11.5)