FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515620
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17250.016.2139511
GTACTAG11200.016.031651
GTCCTAG18750.016.0217861
GTCTTAG17600.015.1067481
ATACGAC4001.2732926E-1114.6672813
GTTCTAA24050.014.3574681
GTATATA26500.014.20278551
GTGCTAG18650.014.0710881
GTACATA20750.013.9782921
GTACTAA16150.013.8974071
GTTCTAG23900.013.7251981
GGTCTAG15400.013.4531341
GCTCTAG22650.013.4155161
CCTAGAC17000.013.1954773
GTATATG26100.013.0974761
GTCCTAA18150.012.9367511
TCTATAC17550.012.5852993
GTATAGG12350.012.5816761
CTATACT17850.012.3715994
GTATAAG16800.012.3320391
Produced by FastQC (version 0.11.5)