FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160268
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG21500.024.865482145
CTGTCTA40700.017.864048145
GTATTAG22000.017.4900281
GTACTAT20150.017.3331221
GTCCTAG25050.016.7783991
GTGCTAG24100.015.2291091
GTATATA32650.015.0485541
GTCCTAA19700.015.0246251
GTACTAA18150.014.6769481
GTACATA24750.014.3507941
GTACTGG42800.014.2460131
GTTCTAA30450.014.1917321
GTATAGG17250.014.0700171
GTATAAG19600.013.8931781
GTTCTAG27050.013.78710651
GTATATG31800.013.5892541
TAGTACC12250.013.5087764
TATACCG5252.4811743E-713.5065835
GTTCATA32300.013.3788941
TTAGACG5353.115547E-713.2562754
Produced by FastQC (version 0.11.5)