FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238464
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA40600.022.527025145
GGGGGGG505100.018.624601145
GTACTAG12450.017.9990961
GTCCTAG22650.016.402451
GTGCTAG24900.014.9203021
GTTCTAG27350.014.8774391
CTATACT22250.014.577034
TCTAGAC20350.014.4891193
GTATAAA39250.014.2731661
GTATTAG19850.014.259921
GTATATA31350.014.1078271
TCTGTAC43650.013.9151973
GTACAGA45950.013.8603641
GTTCTAA27950.013.714121
GTATATG29300.013.6860361
GTATAAG21500.013.1655541
GGTCTAG21100.013.1356561
TAGACTG26000.012.9284035
TTAGACC17800.012.9205494
CCCTTAG24750.012.8663381
Produced by FastQC (version 0.11.5)