FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238464
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG22100.018.509718145
GTATAGG17450.017.6286541
GTCCTAG22050.017.4387761
GTACTAG11350.016.157611
TCTAGAC22900.015.9963343
GGTCTAG17750.015.3310091
CTATACT20450.015.3116654
GTCTTAG23950.015.0673061
TCTATAC21200.015.0495013
GCTCTAG30050.014.961681
GTCTTAA26650.014.8726661
GTTCTAA28800.014.7894241
GTATTAA31600.014.2277991
GTGCTAG24300.013.8764961
GTTCTAG27300.013.8684711
GTACTAT17800.013.6262111
CTATACC17950.013.4945764
GTATTAG20750.013.1144781
GGTCTAA18500.013.1106241
CTAGACT19950.013.0301564
Produced by FastQC (version 0.11.5)