FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064169
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA43950.032.246635145
TAGCGCG9403.6417987E-822.503012145
GGGGGGG430650.019.942062145
GGTCTAA18800.016.360561
GTGCTAG23650.016.2567941
TAGCACG8100.002808306115.668763145
GTCTATA19600.014.787431
GTATTAG20650.014.6084041
TCTATAC20500.014.4271173
GTACTAG11500.014.40166951
GTCCTAG23600.014.286161
GTACAGA45550.014.1543781
CTATACT21400.013.8203694
TCGTATG19950.013.783799145
CCTATAC16350.013.7476763
GCTCTAG28850.013.7366771
GTCCTAA21200.013.3923891
GTATAGG16450.013.3041921
GTCTTAA26850.012.9975191
GTACATA25100.012.9610931
Produced by FastQC (version 0.11.5)