FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064169
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA39300.021.567965145
GTCCTAG24650.017.0407431
GTACTAG11900.016.9035951
CCTGTCG23250.016.405567145
GTCGCTT16953.9053702E-916.25232145
GTCCTAA20650.016.044091
GTATTAA30300.015.6204831
GTTCTAG26650.015.0958941
GGTCTAA18750.014.5145541
GTATAGG16800.014.4384871
GTATTAG18250.013.9396761
TCTAGAC20400.013.9098173
GTATATG29250.013.551771
GTCTTAG19700.013.5142961
CTATACT21900.013.4968574
GTGCTAG23250.013.4865241
GTACTAA19000.013.3894261
CTGTACT45500.013.3823564
GTGTTAG26550.013.3700731
GTACAGA42600.013.1935341
Produced by FastQC (version 0.11.5)